Metal-mediated, autolytic amide bond cleavage: a strategy for the selective, metal complexation-catalyzed, controlled release of metallodrugs

D Śmiłowicz, S Eisenberg, R LaForest… - Journal of the …, 2023 - ACS Publications
… and coworkers employed Ni 2+ chelating peptides that were … Amide bond hydrolysis was
induced by complexation by the … ions, model peptides did not hydrolyze at any temperature …

Refinement of the Fusion Tag PagP for Effective Formation of Inclusion Bodies in Escherichia coli

X Li, B Zhang, Q Hu, C Chen, J Huang, L Liu… - Microbiology …, 2023 - Am Soc Microbiol
… Motif I (ASRHWMAG) allows the fusion proteins to be hydrolyzed site-specifically by Ni 2+ …
II fusion peptide with a sequence (ASRHWMAG) for Ni(II)-dependent peptide bond hydrolysis (…

The past, present, and future of artificial zinc finger proteins: design strategies and chemical and biological applications

S Negi, M Imanishi, M Hamori… - JBIC Journal of …, 2023 - Springer
… of how Ni(II) substitution alters the DNA sequence-specific … Additionally, hydrolysis of peptide
bonds has been reported … in various fields, including protein engineering, medicine, and …

Monodispersed MOF-808 nanocrystals synthesized via a scalable room-temperature approach for efficient heterogeneous peptide bond hydrolysis

S Dai, C Simms, I Dovgaliuk, G Patriarche… - Chemistry of …, 2021 - ACS Publications
… of selective peptide bond and protein hydrolysis among the reported Zr(IV)-based MOFs.
This result may open new opportunities for highly efficient peptide or protein hydrolysis using …

Ni2+-Assisted Hydrolysis May Affect the Human Proteome; Filaggrin Degradation Ex Vivo as an Example of Possible Consequences

EI Podobas, D Gutowska-Owsiak, S Moretti… - Frontiers in Molecular …, 2022 - frontiersin.org
… Its molecular mechanism may include the peptide bond hydrolysis in X 1 -S/Tc/pHcX 2
motifs by released Ni 2+ ions. The goal of the study was to analyse the distribution of such …

DNA Recognition with Artificial Metallopeptides

J Gómez-González, A Alcalde, MV López… - … Protein Engineering …, 2023 - books.google.com
… Despite the advantages of combinatorial DNA binding, the … combining a sequence-specific
DNA-binding peptide with a … This design delivers the hydrolytic ATCUN complex in the …

Non‐Canonical Amino Acids for Engineering Peptides and Proteins with new Functions

KZQ Zhou, R Obexer - Israel Journal of Chemistry, 2024 - Wiley Online Library
… groups, the combinatorial possibilities are astronomical, … applications of ncAAs in peptide
and protein engineering, the … of a metaldependent, sequence-specific DNA endonuclease …

[HTML][HTML] Targeting Ras with protein engineering

A Tomazini, JM Shifman - Oncotarget, 2023 - ncbi.nlm.nih.gov
… Once GTP is hydrolyzed, the affinity of Ras to effectors is … modifications that are
sequence-specific, likely explaining non-… In all protein engineering experiments, large …

Peptide-based supramolecular systems chemistry

F Sheehan, D Sementa, A Jain, M Kumar… - Chemical …, 2021 - ACS Publications
… design aspects and combinatorial dynamic covalent and … ADARYKS that was shown to
have modest, sequence specific … barriers for peptide bond formation and hydrolysis are …

Recent advances in biocatalysis with chemical modification and expanded amino acid alphabet

AD Pagar, MD Patil, DT Flood, TH Yoo… - Chemical …, 2021 - ACS Publications
… for proteins dramatically, which has provided protein engineers … is intercepted by amines to
form amide bonds. Aiming to enhance the … Nevertheless, novel combinatorial strategies were …